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Updated: Feb 10, 2026

Hydrogen Production and Utilization in a Membrane Reactor
Published on: March 10, 2023
Potentials of using mixed culture bacteria incorporated with sodium bicarbonate for hydrogen production from water
Alaa Wazeri1, Mohamed Elsamadony2, Sophie Le Roux3
1Egypt-Japan University of Science and Technology (E-JUST), Environmental Engineering Department, P.O. Box 179, New Borg El-Arab City, Alexandria 21934, Egypt; South Valley University, Faculty of Engineering, Civil Engineering Department, 83523 Qena City, Egypt.
Abstract:
The aim of this study is to assess the potentials of using mixed culture bacteria incorporated with different concentrations of NaHCO3 for hydrogen production from water hyacinth (WH). The lowest hydrogen yield (HY) of 30.4 ± 1.9 mL/gTVS, H2 content (HC) of 19.5 ± 1.5% and hydrogenase enzyme (HE) activity of 0.06 ± 0.01 mgM.Breduced/min were registered for the cultures without supplementation of NaHCO3. The HY, HC, and HE activity were maximized at levels of 69.2 ± 4.3 mL/gTVS, 58.4 ± 3.6% and 0.18 ± 0.01 mgM.Breduced/min. respectively for the anaerobes supplied with 3.0 g NaHCO3/L. Furthermore, cellulose, hemicellulose, and lignin destruction efficiencies were 37.2 ± 2.3, 30.0 ± 1.9 and 20.9 ± 1.3% respectively due to the increase of cellulase and xylanase activities up to 2.73 ± 0.17 and 1.87 ± 0.12 U/mL, respectively. Moreover, the abundance of Firmicutes was substantially increased and accounted for 71% of the total OTU's. Microbes belonging to the order Clostridiales and OPB54 were particularly enriched in the medium supplemented with NaHCO3.
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