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Related Experiment Videos

Plasmin in subretinal fluid.

I J Immonen1, E M Salonen, A I Vaheri

  • 1Department of Ophthalmology, Helsinki University Central Hospital, Finland.

Acta Ophthalmologica
|December 1, 1988
PubMed
Summary

Active plasmin was detected in subretinal fluid of patients with rhegmatogenous retinal detachment. This enzyme may contribute to the development of proliferative vitreoretinopathy by degrading extracellular matrix.

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Area of Science:

  • Ophthalmology
  • Biochemistry

Background:

  • Rhegmatogenous retinal detachment (RRD) is a condition requiring prompt surgical intervention.
  • Proliferative vitreoretinopathy (PVR) is a major cause of RRD treatment failure.

Purpose of the Study:

  • To investigate the presence and activity of plasmin in subretinal fluid (SRF) from patients with RRD.
  • To explore the potential role of plasmin in the pathogenesis of PVR.

Main Methods:

  • Subretinal fluid was collected from 56 eyes undergoing surgery for RRD without hemorrhage.
  • Proteolytic activity, specifically active plasmin and plasmin-inhibitor complexes, was quantified using biochemical assays.

Main Results:

  • Active plasmin was detected in 33 out of 56 eyes (59%), with concentrations ranging from 1.0 to 15.2 micrograms/ml.
  • Plasmin-inhibitor complexes were found in 3 eyes.
  • Plasmin detection was more frequent in larger retinal detachments, but no clear correlation was found with detachment duration or retinal hole characteristics.

Conclusions:

  • Plasmin is present in the subretinal fluid of a significant proportion of RRD patients.
  • Plasmin may contribute to PVR development by degrading the extracellular matrix and facilitating pigment epithelial cell release.
  • Further research is warranted to elucidate the precise role of plasmin in RRD and PVR.

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