Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transcription01:10

Transcription

157.1K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
157.1K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

11.0K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.0K
Master Transcription Regulators02:23

Master Transcription Regulators

7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
Uncertainty in Measurement: Reading Instruments02:46

Uncertainty in Measurement: Reading Instruments

53.6K
Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
53.6K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.9K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.9K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Asynchronous Contralateral Primaries of Small Cell Carcinoma of the Ovary, Hypercalcemic Type in Germline <i>SMARCA4</i> Pathogenic Variant Carriers.

JCO precision oncology·2026
Same author

Single-Cell Spatial Transcriptomics Reveals Sex-Specific Differences Driving Carotid Atherosclerotic Plaque Instability.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Co-occurring rare germline DNA repair gene variants in BRCA1/BRCA2 implicated hereditary breast cancer families.

NPJ breast cancer·2026
Same author

<i>De novo</i> whole-genome assembly of the <i>Wolbachia</i> sp. endosymbiont from <i>Anastrepha fraterculus</i> using long- and short-read metagenomic data.

Microbiology resource announcements·2026
Same author

Benchmarking of sequencing technologies defines optimal strategies for genetic variants detection in a human genome.

Genome biology·2026
Same author

Editorial Expression of Concern: Direct targeting of Sec23a by miR-200s influences cancer cell secretome and promotes metastatic colonization.

Nature medicine·2026

Related Experiment Video

Updated: Feb 10, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

544

Transcript Profiling Using Long-Read Sequencing Technologies.

Anthony Bayega1, Yu Chang Wang1, Spyros Oikonomopoulos1

  • 1Department of Human Genetics, McGill University and Genome Quebec Innovation Centre, McGill University, Montréal, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|May 17, 2018
PubMed
Summary

Long-read RNA sequencing (RNA-Seq) using PacBio and Oxford Nanopore Technologies offers advanced capabilities for de novo transcriptome assembly and isoform quantification. These methods are becoming essential for understanding complex transcriptomes in high-throughput studies.

Keywords:
Long readNanoporeNext-generation sequencingPacBioRNA-SeqTranscriptome

More Related Videos

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

4.2K
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.0K

Related Experiment Videos

Last Updated: Feb 10, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

544
De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

4.2K
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.0K

Area of Science:

  • Genomics
  • Transcriptomics
  • Bioinformatics

Background:

  • Next-generation sequencing (NGS) is standard for gene expression profiling.
  • Short-read RNA sequencing (RNA-Seq) is widely used, but long-read technologies are gaining prominence.
  • The transcriptome's complexity, with variable transcript lengths and alternative splicing, necessitates advanced sequencing approaches.

Purpose of the Study:

  • To detail experimental procedures for PacBio and Oxford Nanopore Technologies (ONT) long-read RNA sequencing.
  • To focus on full-length cDNA synthesis, de novo transcriptome assembly, and isoform quantification.
  • To provide a guide for researchers utilizing long-read RNA-Seq for comprehensive transcriptome analysis.

Main Methods:

  • Library preparation protocols optimized for long-read sequencing (PacBio and ONT).
  • Sequencing strategies tailored for capturing full-length transcripts.
  • Bioinformatic pipelines for de novo transcriptome assembly and accurate isoform quantification.

Main Results:

  • Demonstration of effective full-length cDNA synthesis for diverse transcript lengths.
  • Successful de novo transcriptome assembly revealing complex gene structures.
  • Accurate quantification of alternatively spliced isoforms using long-read data.

Conclusions:

  • Long-read RNA sequencing technologies (PacBio and ONT) are crucial for detailed transcriptome analysis.
  • These methods overcome limitations of short-read sequencing for complex transcriptomes.
  • The described protocols facilitate advanced research in gene expression and isoform diversity.