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Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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What is Gene Expression?01:36

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Chromatin Position Affects Gene Expression02:35

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Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
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Comparative Lesions Analysis Through a Targeted Sequencing Approach
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High Throughput Sequencing-Based Approaches for Gene Expression Analysis.

R Raja Sekhara Reddy1, M V Ramanujam2

  • 1Clevergene Biocorp Private Limited, Bangalore, Karnataka, India.

Methods in Molecular Biology (Clifton, N.J.)
|May 17, 2018
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) provides powerful gene expression analysis for all organisms. This guide simplifies complex RNA-Seq data interpretation for biologists, addressing key challenges in bioinformatics.

Keywords:
Gene expressionNGSRNARNAseqTranscriptome

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Next-generation sequencing (NGS) is a pivotal technology for biological research.
  • RNA-Seq analysis using massively parallel sequencing enhances gene expression understanding in diverse organisms.
  • Data analysis and interpretation present significant challenges despite sequencing advancements.

Purpose of the Study:

  • To discuss the complexities of sequencing and data analysis.
  • To simplify gene expression analysis using sequencing technologies.
  • To provide a practical workflow for biologists and experimental scientists.

Main Methods:

  • Review of next-generation sequencing technologies.
  • Discussion of RNA-Seq data analysis pipelines.
  • Explanation of gene expression pattern interpretation.

Main Results:

  • Sequencing technology is widely accessible (commodity level).
  • Significant challenges exist in analyzing and interpreting large-scale sequencing data.
  • A simplified workflow for RNA-Seq analysis is presented.

Conclusions:

  • NGS is essential for modern biological research.
  • Effective analysis is crucial for leveraging sequencing data.
  • This chapter offers a simplified approach to RNA-Seq for experimental scientists.