Related Experiment Video

Updated: Feb 10, 2026

Activating Autophagy by Aerobic Exercise in Mice
08:44

Activating Autophagy by Aerobic Exercise in Mice

Published on: February 3, 2017

12.8K

Glucocorticoid-activated IRE1α/XBP-1s signaling: an autophagy-associated protective pathway against endotheliocyte

Yanchun Gao1, Hongyi Zhu1, Fan Yang1

  • 1Department of Orthopedic Surgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital , Shanghai , China.

American Journal of Physiology. Cell Physiology
|May 17, 2018
PubMed

Insights

Glucocorticoids cause endoplasmic reticulum stress and autophagy in endotheliocytes. This autophagy, activated by the IRE1α/XBP-1s pathway, protects cells from glucocorticoid-induced damage and apoptosis.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Pathology

Background:

  • Glucocorticoid-induced endothelial injury is a recognized phenomenon in various diseases.
  • The precise molecular mechanisms linking glucocorticoids to endothelial dysfunction remain incompletely understood.
  • Autophagy is a cellular process involved in stress response and cell survival.

Purpose of the Study:

  • To elucidate the role of autophagy in glucocorticoid-induced endotheliocyte injury.
  • To investigate the signaling pathways involved in glucocorticoid-induced autophagy.
  • To determine the functional consequences of autophagy in endotheliocytes under glucocorticoid exposure.

Main Methods:

  • Treatment of endotheliocytes with glucocorticoids.
  • Assessment of apoptosis and endoplasmic reticulum (ER) stress.
  • Analysis of autophagy induction and the involvement of the IRE1α/XBP-1s pathway.
  • Evaluation of cell proliferation and survival.

Main Results:

  • Glucocorticoids induced apoptosis and ER stress in endotheliocytes.
  • Glucocorticoids stimulated autophagy, which was linked to the IRE1α/XBP-1s signaling pathway.
  • Autophagy partially protected endotheliocytes from glucocorticoid-induced apoptosis and proliferation inhibition.

Conclusions:

  • Glucocorticoid-induced ER stress activates the IRE1α/XBP-1s pathway, leading to autophagy.
  • Autophagy plays a protective role in endotheliocyte survival and proliferation against glucocorticoid-induced damage.
Keywords:
IRE1αapoptosisautophagyendoplasmic reticulum stressglucocorticoid

More Related Videos

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR10:54

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Published on: July 27, 2019

9.1K
Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

8.0K

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.6K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.1K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.4K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.5K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.7K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.1K