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Related Experiment Videos

Redefining Molecular Amphipathicity in Reversing the "Coffee-Ring Effect": Implications for Single Base Mutation

Chi Huang1, Jie Wang, Xiaobo Lv

  • 1Institute of Chemical Biology and Nanomedicine, Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering , Hunan University , Changsha 410082 , China.

Langmuir : the ACS Journal of Surfaces and Colloids
|May 22, 2018
PubMed
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Researchers eliminated the coffee ring effect, which causes uneven drying of liquid drops, by controlling molecule properties. This uniform deposition improves biosensing and other applications.

Area of Science:

  • Physical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • The coffee ring effect describes the uneven deposition of solutes from drying sessile drops, forming a ring.
  • This phenomenon negatively impacts applications like biosensing and molecular self-assembly by causing nonuniform material distribution.
  • Controlling solute amphipathicity offers a potential strategy to mitigate this effect.

Purpose of the Study:

  • To demonstrate the elimination of the coffee ring effect by controlling amphipathicity of suspended compounds.
  • To investigate the mechanism of uniform deposition driven by Marangoni flow.
  • To apply this strategy in an enzymatic amplification method for enhanced biosensing.

Main Methods:

  • Modifying DNA with hydrophobic dye and controlling its amphipathicity via nuclease digestion.

Related Experiment Videos

  • Utilizing the induced Marangoni flow in sessile drops for uniform deposition.
  • Implementing the strategy in a nitrocellulose membrane-based biosensing assay.

    • Main Results:

    • Conversion of dye-labeled DNA into amphipathic molecules reversed the coffee ring effect.
    • Uniform disk-shaped deposition of the dye was achieved.
    • The method enhanced assay reliability and sensitivity in a biosensing application.

    Conclusions:

    • Controlling solute amphipathicity effectively eliminates the coffee ring effect.
    • The induced Marangoni flow is key to achieving uniform deposition.
    • This strategy has broad applicability for improving various sessile drop-based systems.