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Updated: Feb 10, 2026

Enhanced Oil Recovery using a Combination of Biosurfactants
Published on: June 3, 2022
Cyclic lipopeptide biosurfactant from Bacillus tequilensis exhibits multifarious activity
Arun Kumar Pradhan1,2, Animesha Rath3, Nilotpala Pradhan4
1Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Bhubaneswar, Odisha 751003 India.
Abstract:
Bacillus tequilensis strain CH had been previously shown to produce a biosurfactant. In this study, chemical structure of the purified biosurfactant was determined by using high performance liquid chromatography and liquid chromatography-mass spectroscopy as a 10 amino acid cyclic lipopeptide (CL). The cyclic lipopeptide was found to be active against Anopheles culicifacies larvae with a LC50 of 110 µg/ml in 2 days. 1 ppm cadmium (Cd) which had a profound mutagenic effect on the cell division of onion (Allium cepa) root tip cell resulting in abnormal metaphase, abnormal anaphase and nuclei elongation was partially reversed in the presence of 0.1 mg/ml of CL (52% cells dividing normally and 8% with abnormal division) and was comparable to control experiment where no Cd was present. Thus, the CL described in this report may have applications in eliminating larvae from water repository systems and in reversing the effects of cadmium pollution.
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