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Related Concept Videos

Constant Volume Calorimetry02:41

Constant Volume Calorimetry

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Calorimeters are useful to determine the heat released or absorbed by a chemical reaction. Coffee cup calorimeters are designed to operate at constant (atmospheric) pressure and are convenient to measure heat flow (or enthalpy change) accompanying processes that occur in solution at constant pressure. A different type of calorimeter that operates at constant volume, colloquially known as a bomb calorimeter, is used to measure the energy produced by reactions that yield large amounts of heat and...
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The volume of a fuel tank mounted on the wing of a jet aircraft can be modeled using the concept of solids of revolution. In this case, the tank is formed by rotating a two-dimensional region, defined by a mathematical function, about the x-axis. The region extends along the axis from zero to two meters, and the resulting three-dimensional shape is symmetric about the axis of rotation. Because the boundary curve lies directly against the axis, the disk method is an appropriate technique for...
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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
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Thrombin generation in low plasma volumes.

Saartje Bloemen1,2, Hilde Kelchtermans1,2, H Coenraad Hemker1,2

  • 1Synapse Research Institute, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands.

Thrombosis Journal
|May 23, 2018
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Summary

Calibrated automated thrombinography for thrombin generation measurement remains accurate when plasma and reagent volumes are halved. Higher volume reductions or plasma dilutions compromise this accuracy.

Keywords:
Calibrated automated thrombinographyLow volumesThrombin generation

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Area of Science:

  • Biochemistry
  • Hematology
  • Clinical Chemistry

Background:

  • Thrombin generation assays are crucial for hemostasis evaluation.
  • Optimizing reagent and plasma volumes can improve assay efficiency and reduce costs.
  • Understanding volume limitations is key for reliable diagnostic testing.

Purpose of the Study:

  • To determine the impact of reduced plasma and reagent volumes on the accuracy of calibrated automated thrombinography.
  • To establish the maximum permissible reduction in volumes for sustained assay precision.

Main Methods:

  • Calibrated automated thrombinography was performed using varying reductions of plasma and reagent volumes.
  • Plasma dilutions were also tested to assess their effect on thrombin generation parameters.
  • Key thrombinography parameters were analyzed to evaluate assay accuracy and reproducibility.

Main Results:

  • Accurate thrombin generation determination was maintained with reductions in plasma and reagent volumes up to 50%.
  • Higher volume reductions (beyond 50%) led to a significant decrease in assay accuracy.
  • Increased plasma dilutions also negatively impacted the reliability of thrombin generation measurements.

Conclusions:

  • Calibrated automated thrombinography can be reliably performed with halved plasma and reagent volumes.
  • Significant reductions in volumes or increased plasma dilutions compromise the accuracy of thrombin generation assays.
  • Assay protocols should adhere to established volume limits to ensure diagnostic validity.