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Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
Published on: April 2, 2020
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Protocols for Migration and Invasion Studies in Prostate Cancer
Arjanneke F van de Merbel1, Geertje van der Horst1, Jeroen T Buijs1
1Department of Urology, Leiden University Medical Center, Leiden, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|May 23, 2018
Summary
Prostate cancer cell migration and invasion are key to metastasis. This study details the Transwell assay, a method to measure these aggressive behaviors in vitro, aiding in understanding cancer progression.
Area of Science:
- Oncology
- Cell Biology
- Cancer Research
Background:
- Prostate cancer metastasis and therapy resistance are significant clinical challenges.
- Cancer cell migration and invasion, including epithelial-to-mesenchymal transition (EMT), are crucial for metastasis.
- In vitro assays are valuable for assessing cancer cell aggressiveness.
Purpose of the Study:
- To describe the Transwell migration assay for prostate cancer cells.
- To detail adaptations of the Transwell assay for studying prostate cancer cell invasion.
- To provide staining and quantification procedures for migration and invasion assays.
Main Methods:
- Utilizing the Transwell migration assay to assess prostate cancer cell motility.
- Adapting the Transwell assay with a matrix coating to evaluate prostate cancer cell invasion.
- Staining Transwell filter inserts for quantitative analysis of cell migration and invasion.
Main Results:
- The Transwell assay effectively measures prostate cancer cell migration in vitro.
- Modified Transwell invasion assays accurately quantify the invasive potential of prostate cancer cells.
- Detailed protocols enable reproducible assessment of migratory and invasive phenotypes.
Conclusions:
- The Transwell migration and invasion assays are essential tools for studying prostate cancer aggressiveness.
- These in vitro methods facilitate research into the mechanisms of prostate cancer metastasis.
- Quantitative analysis of migration and invasion aids in understanding therapeutic resistance.
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