Related Experiment Video
Updated: Feb 10, 2026

MALDI Sample Preparation: the Ultra Thin Layer Method
Published on: April 29, 2007
Detergent-assisted sample preparation for MALDI-MS: Investigation of octylglucoside and docecylmaltoside for matrix
Michael Rühl1, Stefan Schönborn1, Michael Karas1
1Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Max-von-Laue-Straße 9, D-60438, Frankfurt, Germany.
Abstract:
We show an easy and fast method for improved detection of lipophilic peptides with MALDI-MS utilizing the nonionic detergents n-octylglucoside and n-dodecylmaltoside (laurylmaltoside). Investigations comprised on-plate digestion of proteins with trypsin, detergent effects on the protease trypsin, and the changes in MALDI matrix crystallization. Investigations also exhibited a higher tryptic activity in trypsin activity assay of 139% when using laurylmaltoside as supplement. Crystallization changed toward a more homogeneous crystal distribution and especially trypsinized insulin spectra recorded with MALDI-MS showed improved detectability of lipophilic peptides.
Related Concept Videos
Matrix-Assisted Laser Desorption Ionization (MALDI)
Protein Digestion
What is Monogastric Digestion?
Carbohydrate Digestion
Preparation of Samples for Electron Microscopy
Lipid Digestion

