Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

17.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.3K
Synthetic Biology02:55

Synthetic Biology

5.6K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.6K
Synthetic Disvision of Polynomials01:28

Synthetic Disvision of Polynomials

194
Synthetic division is an efficient algorithmic approach for dividing a polynomial by a linear binomial of the form x - c, where c is a real number. This method is helpful due to its streamlined process, which avoids the more cumbersome steps involved in the traditional long division of polynomials. It simplifies computation and serves as a practical tool for evaluating polynomials and identifying their factors.To perform synthetic division, one begins by listing the coefficients of the...
194
Opioid Analgesics: Synthetic and Semisynthetic Opioids01:15

Opioid Analgesics: Synthetic and Semisynthetic Opioids

1.1K
Synthetic and semisynthetic opioids are pivotal in pain management and tackling opioid addiction. Semisynthetic opioids, including morphinans (morphine derivatives), oxycodone, oxymorphone, hydrocodone, and hydromorphone, have improved pharmacokinetic profiles compared to morphine. Additionally, heroin and 6-MAM (6-Monoacetylmorphine) show better CNS penetration than morphine due to heightened lipid solubility. Hydromorphone, a potent opioid, undergoes hepatic metabolism to form the active...
1.1K
Identifying Statistically Significant Differences: The F-Test01:14

Identifying Statistically Significant Differences: The F-Test

3.9K
The F-test is used to compare two sample variances to each other or compare the sample variance to the population variance. It is used to decide whether an indeterminate error can explain the difference in their values. The underlying assumptions that allow the use of the F-test include the data set or sets are normally distributed, and the data sets are independent of each other. The test statistic F is calculated by dividing one variance by another. In other words, the square of one standard...
3.9K
Design Example: Identifying the Locations of Monuments in the Field Using Global Positioning System Device01:30

Design Example: Identifying the Locations of Monuments in the Field Using Global Positioning System Device

410
Surveyors use Global Positioning System (GPS) technology to measure the precise location and elevation of points on Earth. In a recent survey, GPS receivers were used to determine the coordinates and elevations of two park monuments. The process involved careful mission planning, data collection, and correction to ensure accuracy. The survey began with mission planning to identify optimal satellite visibility and minimize Position Dilution of Precision (PDOP). A geodetic control point...
410

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards the construction of a virtual yeast.

Nature·2026
Same author

Bioremediation of complex organic pollutants by engineered Vibrio natriegens.

Nature·2025
Same author

A new set of mutations in the second transmembrane helix of the Cox2p-W56R substantially improves its allotopic expression in Saccharomyces cerevisiae.

Genetics·2025
Same author

Towards next-generation DNA encryption via an expanded genetic system.

National science review·2025
Same author

Decoding genetic diversity through genome engineering in bryophytes.

The Plant journal : for cell and molecular biology·2025
Same author

SCRaMbLE: a Versatile Tool for Genome Manipulation.

Physiology (Bethesda, Md.)·2025

Related Experiment Video

Updated: Feb 10, 2026

A Data Integration Workflow to Identify Drug Combinations Targeting Synthetic Lethal Interactions
07:40

A Data Integration Workflow to Identify Drug Combinations Targeting Synthetic Lethal Interactions

Published on: May 27, 2021

4.6K

Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES.

Zhouqing Luo1,2, Lihui Wang2, Yun Wang3,4

  • 1Center for Synthetic Biology Engineering Research, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Nature Communications
|May 24, 2018
PubMed
Summary

We developed ReSCuES, a reporter for SCRaMbLE (synthetic chromosome Rearrangement and Modification by LoxP-mediated Evolution) in yeast. This system efficiently identifies strains with beneficial genetic rearrangements for improved stress tolerance.

More Related Videos

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
07:56

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Published on: January 17, 2012

29.3K
Mating-based Overexpression Library Screening in Yeast
11:39

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

8.2K

Related Experiment Videos

Last Updated: Feb 10, 2026

A Data Integration Workflow to Identify Drug Combinations Targeting Synthetic Lethal Interactions
07:40

A Data Integration Workflow to Identify Drug Combinations Targeting Synthetic Lethal Interactions

Published on: May 27, 2021

4.6K
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
07:56

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Published on: January 17, 2012

29.3K
Mating-based Overexpression Library Screening in Yeast
11:39

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

8.2K

Area of Science:

  • Synthetic biology
  • Genomics
  • Yeast genetics

Background:

  • SCRaMbLE enables massive chromosome rearrangements in synthetic yeast genomes, generating genotypic diversity.
  • Efficient selection methods are crucial for isolating SCRaMbLEd strains with desired traits.

Purpose of the Study:

  • To develop and validate ReSCuES, a reporter system for efficient selection of SCRaMbLEd yeast strains.
  • To demonstrate the utility of ReSCuES in identifying strains with enhanced stress tolerance.
  • To investigate the genetic basis of stress tolerance in SCRaMbLEd strains.

Main Methods:

  • Implementation of ReSCuES, a loxP-mediated switch reporter for auxotrophic markers.
  • Induction of SCRaMbLE to generate chromosomal rearrangements in synthetic yeast.
  • Selection and isolation of SCRaMbLEd clones using the ReSCuES system.
  • Phenotypic analysis of selected strains for tolerance to ethanol, heat, and acetic acid.
  • Genetic analysis to identify key regulators of stress tolerance.

Main Results:

  • ReSCuES demonstrated high efficiency in selecting SCRaMbLEd yeast strains, with all isolated clones exhibiting chromosomal rearrangements.
  • SCRaMbLE, facilitated by ReSCuES, generated strains with significantly increased tolerance to ethanol, heat, and acetic acid.
  • Identification of ACE2, a transcription factor, as a negative regulator of ethanol tolerance.

Conclusions:

  • ReSCuES provides a generic and efficient platform for rapidly identifying yeast strains of interest generated by SCRaMbLE.
  • This study establishes a method to dissect the genetic mechanisms underlying stress resistance in engineered yeast.
  • The findings highlight the potential of synthetic genomics approaches for strain improvement and biological discovery.