Microbial Load of Trocars: Potential Source of Contamination and Surgical Site Infection

Area of Science:

• Gynecological Surgery
• Infectious Disease Epidemiology
• Microbiology

Background:

• Surgical site infection (SSI) is a significant cause of morbidity, mortality, and increased healthcare costs.
• Understanding the microbial dynamics at surgical sites is crucial for preventing SSIs in laparoscopic gynecological surgery.

Purpose of the Study:

• To investigate the correlation between the microbial load on surgical trocars, the surgical site microbiota, and the incidence of SSIs.
• To assess the effectiveness of skin antisepsis and trocar disinfection protocols in laparoscopic gynecological procedures.

Main Methods:

• Prospective longitudinal study involving 24 patients undergoing laparoscopic gynecological surgery.
• Collection of swab samples from surgical sites (umbilicus, vaginal fornix, skin) and trocars.
• Microbiological analysis to determine microbial load and identify microorganisms.

Main Results:

• Microorganisms were detected in 100% of umbilicus and vaginal fornix swabs.
• Bacterial growth was absent in 87.5% of trocar samples, indicating effective disinfection.
• Skin antisepsis proved effective in decolonizing the surgical field.

Conclusions:

• Despite microbial presence at surgical sites, meticulous trocar disinfection and skin antisepsis are effective in preventing SSIs.
• Adherence to sterile techniques is paramount in minimizing infection risks during laparoscopic gynecological surgery.