Microbial Load of Trocars: Potential Source of Contamination and Surgical Site Infection

Vanessa Aparecida Vilas-Boas1, Carlos Emílio Levy2, Maria Isabel Pedreira de Freitas3

  • 1Women's Health Hospital "Prof Dr José Aristodemo Pinotti" (CAISM), University of Campinas (UNICAMP), Campinas/SP - Brazil.

Insights

This study found that while surgical sites in gynecological procedures often harbor microorganisms, properly disinfected trocars and effective skin antisepsis significantly reduce infection risks. This highlights the importance of sterile techniques in preventing surgical site infections (SSI).

Area of Science:

  • Gynecological Surgery
  • Infectious Disease Epidemiology
  • Microbiology

Background:

  • Surgical site infection (SSI) is a significant cause of morbidity, mortality, and increased healthcare costs.
  • Understanding the microbial dynamics at surgical sites is crucial for preventing SSIs in laparoscopic gynecological surgery.

Purpose of the Study:

  • To investigate the correlation between the microbial load on surgical trocars, the surgical site microbiota, and the incidence of SSIs.
  • To assess the effectiveness of skin antisepsis and trocar disinfection protocols in laparoscopic gynecological procedures.

Main Methods:

  • Prospective longitudinal study involving 24 patients undergoing laparoscopic gynecological surgery.
  • Collection of swab samples from surgical sites (umbilicus, vaginal fornix, skin) and trocars.
  • Microbiological analysis to determine microbial load and identify microorganisms.

Main Results:

  • Microorganisms were detected in 100% of umbilicus and vaginal fornix swabs.
  • Bacterial growth was absent in 87.5% of trocar samples, indicating effective disinfection.
  • Skin antisepsis proved effective in decolonizing the surgical field.

Conclusions:

  • Despite microbial presence at surgical sites, meticulous trocar disinfection and skin antisepsis are effective in preventing SSIs.
  • Adherence to sterile techniques is paramount in minimizing infection risks during laparoscopic gynecological surgery.

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