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Updated: Feb 10, 2026

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
Published on: July 11, 2025
Immunodiagnosis in osteoarticular tuberculosis by ELISA
1Department of Orthopaedic Surgery, Mahatma Gandhi Institute of Medical Sciences, Wardha, Sevagram 442102, Maharashtra, India.
Background:
It was previously difficult to confirm a clinical diagnosis of tuberculosis by a cheap test, but recently ELISA for circulating antibodies has been found to be reliable in certain cases. We have used this test in osteoarticular tuberculosis.
Methods:
We studied 50 patients with the disease (34 confirmed by biopsy and 16 who responded to chemotherapy) and compared them with 50 matched control subjects. ELISA was performed using Antigen 60-a cell wall cytoplasmic antigen of Mycobacterium tuberculosis.
Results:
The test was positive in 27 out of 34 biopsy proven cases, 11 out of 16 patients who responded to chemotherapy and in only 1 out of 50 control subjects.
Conclusions:
ELISA using Antigen 60 may be a useful confirmatory test for osteoarticular tuberculosis.
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