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Related Concept Videos

Lysosomes01:31

Lysosomes

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Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
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Lysosomal Hydrolases01:22

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Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
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Delivery Pathways to the Lysosome01:36

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Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
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Self-Evaluation: Self-Enhancement and Self-Verification03:00

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Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
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Bioavailability Enhancement: Drug Solubility Enhancement01:16

Bioavailability Enhancement: Drug Solubility Enhancement

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Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
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Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

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Related Experiment Video

Updated: Feb 10, 2026

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
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Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

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Docetaxel enhances lysosomal function through TFEB activation.

Jianbin Zhang1, Jigang Wang2,3, Yin Kwan Wong4

  • 1Department of Oncology, Clinical Research Institute, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China. zhangjianbin@hmc.edu.cn.

Cell Death & Disease
|May 26, 2018
PubMed
Summary
This summary is machine-generated.

Docetaxel enhances cancer cell autophagy and lysosomal function, mediated by transcription factor EB and reactive oxygen species. Inhibiting lysosomes increases docetaxel

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Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Docetaxel is a widely used chemotherapy agent for cancer treatment.
  • Autophagy plays a role in docetaxel's anticancer effects, but its impact on lysosomal function is unclear.

Purpose of the Study:

  • To investigate the effect of docetaxel on lysosomal function and its underlying mechanisms in cancer cells.
  • To explore the potential of targeting lysosomal function in docetaxel-based cancer therapy.

Main Methods:

  • Docetaxel treatment of various cancer cell lines.
  • Analysis of autophagic flux and lysosomal activity.
  • Assessment of transcription factor EB (TFEB) nuclear translocation and transcriptional activity.
  • Investigation of reactive oxygen species (ROS) involvement.
  • Evaluation of cell death upon lysosomal inhibition.

Main Results:

  • Docetaxel enhances autophagic flux, lysosomal function, and autophagosome-lysosome fusion.
  • Docetaxel activates TFEB, promoting lysosome biogenesis and function, which is ROS-dependent.
  • Inhibition of lysosomal function potentiates docetaxel-induced cancer cell death.

Conclusions:

  • Docetaxel activates TFEB-mediated lysosomal function via ROS generation.
  • Lysosomal activation appears to protect cancer cells from docetaxel-induced apoptosis.
  • Targeting lysosomal function could be a novel strategy to enhance docetaxel efficacy in cancer treatment.