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Published on: April 1, 2019
Genetic polymorphism in RANK is associated with mandibular size
Erika Calvano Küchler1, Mariele Andrade do Nascimento1, Mirian Aiko Nakane Matsumoto2
1a Department of Pediatric Dentistry, School of Dentistry of RibeirãoPreto , University of São Paulo , RibeirãoPreto , Brazil.
Objective:
The aim of this study was to evaluate the association between genetic polymorphisms in RANK, RANKL and OPG with maxillary and mandibular dimensions in humans.
Design:
DNA extracted from saliva and the rs3826620, rs9594738 and rs2073618 polymorphisms in RANK, RANKL and OPG, respectively, were analysed by real-time PCR. Four linear measurements (Co-Gn, GoPg, Co-Go and PTM-A) from lateral cephalograms were examined for the evaluation of craniofacial measurements. ANOVA testing and a multivariate linear regression analysis, adjusted for age and gender, were used for statistical analysis, with an alpha of 5%. Hardy-Weinberg equilibrium was also evaluated using the chi-square test within each polymorphism.
Setting:
School of Dentistry of Ribeirão Preto, University of São Paulo.
Participants:
A total of 100 unrelated non-syndromic Brazilian Caucasian subjects were included in this study.
Results:
The polymorphism in RANK was associated with a higher Go-Pg measurement (p = .039). In the multivariate analysis, adjusted for age and gender, the polymorphism in RANK was associated with Go-Pg (p = .017) and Co-Gn (p = .043).
Conclusion:
The polymorphism rs3826620 in RANK is associated with the mandibular size.
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