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Updated: Feb 10, 2026

In situ TEM of Biological Assemblies in Liquid
Published on: December 30, 2013
Double-tilt in situ TEM holder with ultra-high stability
Mingjie Xu1, Sheng Dai1, Thomas Blum2
1Department of Chemical Engineering and Materials Science, University of California Irvine, Irvine, CA 92697, United States.
Abstract:
A double tilting holder with high stability is essential for acquiring atomic-scale information by transmission electron microscopy (TEM), but the availability of such holders for in situ TEM studies under various external stimuli is limited. Here, we report a unique design of seal-bearing components that provides ultra-high stability and multifunctionality (including double tilting) in an in situ TEM holder. The seal-bearing subsystem provides superior vibration damping and electrical insulation while maintaining excellent vacuum sealing and small form factor. A wide variety of in situ TEM applications including electrical measurement, STM mapping, photovoltaic studies, and CL spectroscopy can be performed on this platform with high spatial resolution imaging and electrical sensitivity at the pA scale.
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