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Automated analysis for differentiating leukocytes in body fluids using the software "biological liquid application"
Alessandra Falda1, Paolo Doretto2
1Laboratorio Analisi, ULSS7 Pedemontana, Santorso, Itaty.
Introduction:
We evaluated the "Biological liquid application ADVIA2120" software for differentiating the percentage of polymorphonucleated (%PMN) and mononucleated cells (%MN) in ascitic, pleural, and peritoneal dialysis (PD) fluid.
Methods:
Biological fluid test results of 193 specimens obtained by automated methods (87 with and 106 without dedicated software) were compared with May-Grünwald-Giemsa (MGG) stained blood smears. Limit of detection (LoD) and quantitation (LoQ), repeatability, and inaccuracy were assessed.
Results:
Good agreement between the automated methods with dedicated software and the manual method for %PMN and %MN was obtained for leukocyte differentiation in ascitic and pleural fluids, while correlation with the manual method for PD fluid was poor, both with and without the dedicated software.
Conclusions:
We demonstrated that the automated differentiation of leukocytes with dedicated software on the ADVIA2120 analyzer for body fluids is a good alternative to the microscopic reference method for peritoneal and pleural specimens, but not for PD fluids.
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