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Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
Published on: September 15, 2016
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Label-free microarray-based detection of autoantibodies in human serum
Joseph Bucukovski1, Jared A Carter1, Christopher C Striemer2
1Adarza BioSystems, Inc., 2184 Welsch Industrial Court, St. Louis, MO 63146, United States.
Journal of Immunological Methods
|May 27, 2018
Summary
This study introduces a novel label-free Arrayed Imaging Reflectometry (AIR) platform for multiplex autoantibody detection. The AIR technology enables rapid, sensitive, and quantitative analysis of multiple autoantigens in a single assay, comparable to traditional methods.
Area of Science:
- Immunology
- Biotechnology
- Analytical Chemistry
Background:
- Multiplex autoantibody assays are crucial for autoimmune disease research and clinical diagnostics.
- Current methods can be complex and time-consuming.
- Label-free technologies offer potential for streamlined assay development and workflow.
Purpose of the Study:
- To fabricate and evaluate a 5-plex autoantigen microarray using the Arrayed Imaging Reflectometry (AIR) platform.
- To demonstrate the capability of AIR for label-free, multiplex detection of autoantibodies.
- To compare AIR performance against established singleplex ELISA and Luminex assays.
Main Methods:
- Fabrication of a 5-plex autoantigen microarray.
- Utilizing the Arrayed Imaging Reflectometry (AIR) platform for label-free detection.
- Testing with single-donor human serum samples.
- Comparison with singleplex ELISA and Luminex assay results.
Main Results:
- Successful fabrication and testing of the 5-plex autoantigen microarray.
- Demonstrated rapid, sensitive, and quantitative detection of autoantibodies using AIR.
- AIR achieved multiplex results comparable to singleplex ELISA and Luminex assays for Ro60, La/SSB, Scl-70, BicD2, and Ro52 antibodies.
- The AIR platform functions in a standard multiwell format.
Conclusions:
- The Arrayed Imaging Reflectometry (AIR) platform is a viable label-free technology for multiplex autoantibody detection.
- AIR offers a streamlined approach for assay development and workflow in autoimmune diagnostics.
- AIR provides comparable sensitivity and accuracy to existing multiplex assays.
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