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Updated: Feb 10, 2026

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System
Published on: December 16, 2019
Comparison of illumination geometry for lifetime-based measurements in whole-body preclinical imaging
Nattawut Sinsuebphon1, Alena Rudkouskaya2, Margarida Barroso2
1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York.
Abstract:
Macroscopic fluorescence lifetime imaging (MFLI) has been proved to be an accurate tool to quantify Förster resonance energy transfer (FRET) lifetime-based assessment of receptor-ligand engagement in vitro and in vivo. Herein, we report on the quantitative comparison of MFLI for whole-body preclinical studies in transmittance and reflectance geometries. The comparative study was conducted for both in vitro and in vivo conditions. FRET quantification performance in both geometries was similar in high fluorescence concentration samples. However, the reflectance geometry performed better at low fluorescence concentration. In addition, reflectance geometry could acquire subsurface imaging of the main whole-body organs of small animals without being compromised by tissue attenuation.
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