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Related Experiment Video

Updated: Feb 10, 2026

Tickling, a Technique for Inducing Positive Affect When Handling Rats
05:37

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Published on: May 8, 2018

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Tickling, a Technique for Inducing Positive Affect When Handling Rats.

Sylvie Cloutier1, Megan R LaFollette2, Brianna N Gaskill2

  • 1Center for the Study of Animal Well-being, Department of Integrative Physiology and Neuroscience, Washington State University; Canadian Council on Animal Care; scloutier@ccac.ca.

Journal of Visualized Experiments : Jove
|May 29, 2018
PubMed
Summary

Tickling laboratory rats using a standardized playful handling technique significantly reduces their fear of humans. This method improves animal welfare and facilitates handling for research and husbandry, while modeling positive affective states.

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Area of Science:

  • Animal Behavior
  • Laboratory Animal Science
  • Animal Welfare

Background:

  • Handling laboratory rats can induce fear, stress, and reduced welfare.
  • Current habituation methods are often ineffective and variable.
  • Fearful rats present challenges for research and husbandry.

Purpose of the Study:

  • To describe a standardized rat tickling technique.
  • To investigate its effectiveness in reducing fear and improving welfare.
  • To explore its utility in research and social enrichment.

Main Methods:

  • A systematic, playful handling technique ('tickling') mimicking rat social play was developed.
  • The method was applied to laboratory rats.
  • Behavioral responses and handling ease were assessed.

Main Results:

  • Tickling consistently elicited positive behavioral responses in rats.
  • The technique effectively reduced rats' fearfulness of humans.
  • Improved handling for husbandry and research procedures was observed.

Conclusions:

  • Standardized rat tickling is an effective and practical method to reduce fear and enhance welfare.
  • This technique can serve as a social enrichment and aid in research.
  • It reliably models positive affective states in laboratory rats.