Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Natural killer cell-mediated lysis involves an hydroxyl radical-dependent step.

A K Duwe, J Werkmeister, J C Roder

    Journal of Immunology (Baltimore, Md. : 1950)
    |April 1, 1985
    PubMed
    Summary

    Human natural killer (NK) cells use hydroxyl radicals to lyse target cells. Inhibiting these radicals significantly reduces NK cell cytotoxicity, indicating their crucial role in cell killing.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Do NK cells play a role in anti-tumor surveillance?

    Immunology today·2014
    Same author

    The production of monoclonal antibodies from human lymphocytes.

    Immunology today·2014
    Same author

    DISC1 as a genetic risk factor for schizophrenia and related major mental illness: response to Sullivan.

    Molecular psychiatry·2014
    Same author

    Attenuation of mania-like behavior in Na(+),K(+)-ATPase α3 mutant mice by prospective therapies for bipolar disorder: melatonin and exercise.

    Neuroscience·2013
    Same author

    Genetic suppression of agrin reduces mania-like behavior in Na+ , K+ -ATPase α3 mutant mice.

    Genes, brain, and behavior·2012
    Same author

    The partial isolation of subcellular MHC products which are recognized by alloimmune T lymphocytes.

    Immunogenetics·2012

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Natural killer (NK) cells are crucial for innate immunity.
    • NK cell-mediated cytotoxicity involves various molecular mechanisms.
    • The precise role of oxygen radicals in NK cell lysis requires further elucidation.

    Purpose of the Study:

    • To investigate the involvement of oxygen radicals, specifically hydroxyl radicals, in the lysis of K562 target cells by human NK cells.
    • To determine the source and mechanism of hydroxyl radical generation during NK cell-mediated cytotoxicity.

    Main Methods:

    • Utilized scavengers of different oxygen radicals (superoxide anions, hypochlorite, hydroxyl radicals) to assess their impact on NK cell lysis.
    • Assessed NK cell toxicity under hypoxic conditions.

    Related Experiment Videos

  • Evaluated the effect of scavengers on target cell binding and NK cell morphology.
  • Investigated the role of the lipoxygenase pathway using specific inhibitors.

    • Main Results:

    • Hydroxyl radical scavengers significantly inhibited NK cell-mediated lysis of K562 cells.
    • Inhibition correlated directly with the scavengers' rate constants for hydroxyl radical reaction.
    • Superoxide anion and hypochlorite scavengers had no significant effect on lysis.
    • Target cell binding and NK cell morphology remained unaffected by inhibitory concentrations of scavengers.
    • Inhibitors of the lipoxygenase pathway also reduced NK cell lysis, suggesting it as a potential source of hydroxyl radicals.

    Conclusions:

    • Hydroxyl radicals are essential mediators of human NK cell-induced target cell lysis.
    • NK cell-mediated cytotoxicity is dependent on the generation of hydroxyl radicals.
    • The lipoxygenase pathway may be involved in producing the hydroxyl radicals required for NK cell cytotoxic activity.