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Updated: Feb 9, 2026
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A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
Published on: March 3, 2016
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Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines
Yixiang Zhong1, Taewoong Choi1, Minjae Kim1
1Department of Molecular & Life Science, College of Science and Technology, Hanyang University (ERICA Campus), 55 Hanyangdaehak-ro, Sangrok-gu, Ansan-si, Gyeonggi-do 15588, Republic of Korea.
Stem Cell Research
|May 30, 2018
Summary
Researchers developed new mouse primitive XEN (pXEN) cells from blastocysts. These pXEN cells are more representative of early development than existing XEN cells and are homologous to rat XENP cells.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Genetics
Background:
- Mouse blastocysts possess extraembryonic endoderm (ExEn) precursors with lineage plasticity, Oct4 expression, and Jak/Stat signaling dependence.
- Existing rat ExEn precursor-like cell lines (XENP cells/HypoSCs) share these traits, but equivalent mouse cell lines were previously unavailable.
Purpose of the Study:
- To establish and characterize novel mouse blastocyst-derived cell lines representing ExEn precursors.
- To compare these new cell lines with existing mouse XEN cells and rat XENP cells/HypoSCs.
Main Methods:
- Derivation of primitive XEN (pXEN) cell lines from mouse blastocysts.
- Comparative analysis of pXEN cells, established mouse XEN cells, and rat XENP cells/HypoSCs.
- Assessment of morphology, differentiation potential, gene expression profiles, and lineage plasticity.
Main Results:
- Novel mouse blastocyst-derived pXEN cells were established, exhibiting key ExEn precursor characteristics.
- pXEN cells share more features with rat XENP cells/HypoSCs than previously described mouse XEN cell lines.
- pXEN cells demonstrate greater similarity to the blastocyst stage compared to XEN cells and can readily convert to XEN-like cells.
Conclusions:
- pXEN cells are a more accurate model for blastocyst-stage ExEn precursors than current mouse XEN cells.
- Mouse pXEN cells are the functional homologs of rat XENP cells/HypoSCs, suggesting a reclassification to rat pXEN cells.
- These findings provide a valuable new tool for studying early extraembryonic lineage development in mice.
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