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A case control study on HDL associated PON1 enzyme level in Northern Indian type 2 diabetes mellitus patients
Mohd Wamique1, Wahid Ali2, D Himanshu Reddy3
1HPLC Lab, Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh 226003, India; Department of Biotechnology, Dr. A. P. J. Abdul kalam Technical University, Lucknow, Uttar Pradesh 226003, India.
Aim:
To evaluate the serum paraoxonase 1 activity and determine its association with duration in type 2 Diabetes mellitus patients.
Methods:
A total of 80 cases from type 2 diabetes mellitus and healthy controls were enrolled in the present case control study. Human serum PON1 concentration was measured by ELISA and western blotting and it activity was determined spectrophotometrically using 4-nitrophenyle acetate. Diagnostic accuracy of serum PON1 to identify type 2 Diabetes mellitus was calculated with ROC analysis.
Result:
Serum concentration of LDL, VLDL, TG, A1C, FBS and TC levels showed significantly higher levels in type 2 diabetes patients as compared to healthy controls, however there were no significant differences found in the level of HDL. Serum PON1 concentration and activity monitored in patients with >1 year diabetes showed higher level (75.1 ± 6.8 ng/mL) as compared to patients with >3 years diabetes (65.24 ± 1.6 ng/mL), its level was further decreased in patients with >5 (53.8 ± 2.6 ng/mL) and >7 years (48.1 ± 2.7 ng/mL) of diabetes. PON1 concentration decreased as the duration of diabetes increased. PON1 level was further decreased due to habits like smoking and alcohol consumption.
Conclusion:
Serum PON1 levels decrease in states of high oxidative stress like metabolic syndrome, obesity, uncontrolled diabetes, and dyslipidemia. It can be used as diagnostic marker for diabetes mellitus along with increased TG, LDL, VLDL and FBG.
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