Evaluation of the Wound Healing Activity of a Traditional Compound Herbal Product Using Rat Excision Wound Model

Iranian Traditional Medicine (ITM) manuscripts contain prescriptions that have long been used for healing of wounds. The present study evaluates the healing effect of a poly herbal paste (PHP), retrieved from ITM sources, containing Aloe vera, Commiphora myrrha and Boswellia carteri using rat excision wound model. Excision wounds were induced in six groups consisting of six rats each. Group 1 received no treatment, while groups 2 and 6 received tetracycline ointment, Alpha ointment, PHP 40%, PHP 10% and paste base every day, respectively. The percentage of wound contraction on days 2, 7, 14 and 21 and histopathology parameters of healed wounds on 14^th and 21^st days were evaluated. Moreover, antioxidant activity of PHP was evaluated using DPPH method. There was a significant improvement in wound healing in PHP 10% group on the 7^th day of the treatment (p<0.05). Moreover, the healing effect of PHP 10% was significantly greater than the control, tetracycline and paste base groups on the 2^nd, 14^th and 21^st days (p<0.05). On day 14, PHP 40% showed significant healing effect compared to the control, tetracycline and paste base groups (p<0.05). Fewer inflammatory cells were observed in PHP 10%-treated animals and this group demonstrated better re-epithelialization with remarkable neovascularization. Besides, the PHP 10% formulation exhibited antioxidant activity. In vivo and histopathologic examinations showed considerable wound healing in PHP 10% group. This finding could probably be due to the antioxidant, anti-inflammatory and antimicrobial activities of phytoconstituents of A. vera, B. carteri and C. myrrha.