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Updated: Feb 9, 2026

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
Published on: April 22, 2019
[Evaluation of Material Permeability of Type I Collagen Hydrogel]
Xiaojie Duan1, Shan Liu2, Yue Ma3
1National Institutes for Food and Drug Control, Beijing, 102629.
Objectives:
To establish an experimental method for evaluating material permeability of type I collagen hydrogels.
Methods:
Using BSA-FITC as an indicator, by combining BSA-FITC with PBS they were used as permeability media, and using transwell load hydrogen sample to detect BSA-FITC transparent rate.
Results:
In the concentration range of 100 μg·mL-1~0.781 μg·mL-1, the standard curve R2 ≥ 0.99, Lower Limit of Quantity (LLOQ) is 3.125 μg·mL-1, RSD <5%, detection recovery rate is in the range of 80%~120%.
Conclusions:
In this study, we established an experimental method for evaluating material permeability of hydrogel. The BSA-FITC transparent rate of type I collagen hydrogel was 100% at 28 h.
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