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Updated: Jan 31, 2026

Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation
Published on: June 30, 2023
Small-Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes
Mitsuhiro Kimura1, Sachiko Isobe2
1Enzyme Research Team, Biomass Engineering Research Division, RIKEN Center for Sustainable Resource Science, Wako, Saitama, Japan. mitsuhiro.kimura@riken.jp.
Abstract:
Agrobacterium-mediated transformation is a powerful strategy for plant genetic engineering. However, the transformation efficiency of some grain legume crops is generally low. In this chapter, we describe a chemical screening procedure for improving the efficiency of Agrobacterium-mediated transformation of a model legume, Lotus japonicus. Moreover, we explain the transformation protocol using chloroxynil, a phenolic compound that improves the efficiency.
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