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Updated: Feb 9, 2026

Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess
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Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess

Published on: June 1, 2022

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Mitochondrial Permeability Transition Pore and Calcium Handling

Randi J Parks1, Elizabeth Murphy1, Julia C Liu2

  • 1Systems Biology Center, NHLBI, NIH, Bethesda, MD, 20892, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 1, 2018
PubMed

Insights

The mitochondrial permeability transition pore (PTP) opening causes heart cell death during ischemia-reperfusion injury. PTP inhibitors and strategies reducing PTP triggers offer cardioprotection against this injury.

Area of Science:

  • Cardiovascular Biology
  • Mitochondrial Physiology
  • Cell Death Mechanisms

Background:

  • The mitochondrial permeability transition pore (PTP) is a key channel in the inner mitochondrial membrane.
  • PTP opening is a primary mediator of cell death in cardiac ischemia-reperfusion (I/R) injury.
  • Cardioprotective strategies often target PTP modulation or its triggers.

Purpose of the Study:

  • To review key issues in the study of the PTP.
  • To present methods for measuring PTP opening in isolated mitochondria.
  • To highlight the role of PTP in cardiac I/R injury.

Main Methods:

  • Review of existing literature on PTP and cardiac I/R injury.
  • Description of techniques for assessing PTP opening in isolated mitochondria.

Main Results:

  • PTP opening is a critical event in the pathogenesis of cardiac I/R injury.
  • Inhibitors of PTP reduce cardiac I/R injury in animal models.
  • Cardioprotection is achieved by reducing PTP triggers (e.g., Ca2+, ROS) or inhibiting PTP modulators.

Conclusions:

  • The PTP is a significant therapeutic target for preventing cardiac cell death.
  • Understanding PTP mechanisms is crucial for developing effective cardioprotective therapies.
  • Methods for measuring PTP opening are essential for research and drug development.
Keywords:
CalciumMitochondriaPore openingSwellingUptake

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