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Visual and Microscopic Evaluation of Streptomyces Developmental Mutants
Published on: September 12, 2018
Streptomyces aqsuensis sp. nov., isolated from ditch sediment in Xinjiang, China
Jianming Wang1, Qiaoyan Zhang1, Yang Wang1
1Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps/College of Life Science, Tarim University, Alar 843300, PR China.
Abstract:
A novel actinobacterium, designated TRM46399T, was isolated from ditch sediment in Xinjiang Province, Northwest China. Phylogenetic, physiological, chemotaxonomic and morphological analyses demonstrated that strain TRM46399T belonged to the genus Streptomyces. The strain was aerobic, Gram-stain-positive, had aerial mycelia that branched monopodially and formed chains of arthrospores. The spores were oval to cylindrical with smooth surfaces. The strain had an optimum NaCl concentration for growth of 5 % (w/v). The whole-cell sugar pattern of TRM46399T consisted of ribose, xylose, mannose and galactose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H10). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Major fatty acids were iso-C16 : 0 (47.8 %), iso-C14 : 0 (13.5 %), C16 : 0 (8.9 %), anteiso-C15 : 0 (8.4 %), anteiso-C17 : 0 (4.2 %) and C17 : 0cyclo (2.7 %). The G+C content of the genomic DNA was 68.7 mol%. Comparative 16S rRNA gene sequence analysis indicated that strain TRM46399T (KY688182) was phylogenetically most closely related to Streptomyces xinghaiensis S187T (99.13 % sequence similarity); however, DNA-DNA hybridization studies between stain TRM46399T and S. xinhaiensis S187T showed only 65.32 % relatedness. Based on the evidence from this polyphasic study, strain TRM46399T represents a novel species of the genus Streptomyces, for which the name Streptomyces aqsuensis sp. nov. is proposed. The type strain is TRM46399T (=KCTC 39610 T=CCTCC AA 2015009T).
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