Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

From DNA to Protein03:06

From DNA to Protein

22.5K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.5K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K
Protein and Protein Structure02:15

Protein and Protein Structure

88.4K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
88.4K
Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
What are Proteins?01:55

What are Proteins?

240.0K
Overview
240.0K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.7K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Membrane-associated DELLA degradation modulates growth under carbon/nitrogen imbalance.

The Plant cell·2026
Same author

Signalling and regulation of plant development by carbon/nitrogen balance.

Physiologia plantarum·2025
Same author

Workflow to Select Functional Promoter DNA Baits and Screen Arrayed Gene Libraries in Yeast.

Current protocols·2024
Same author

Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1.

The New phytologist·2024
Same author

Seed Longevity and Ageing: A Review on Physiological and Genetic Factors with an Emphasis on Hormonal Regulation.

Plants (Basel, Switzerland)·2024
Same author

Citrate-Citric Acid RNA Isolation (CiAR) for Fast, Low-Cost, and Reliable RNA Extraction from Multiple Plant Species and Tissues.

Current protocols·2021

Related Experiment Video

Updated: Feb 9, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

18.6K

Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins.

Rocío Sánchez-Montesino1, Luis Oñate-Sánchez2

  • 1Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, Madrid, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|June 2, 2018
PubMed
Summary

This study presents a simple, high-throughput yeast hybrid screening protocol for DNA-protein and protein-protein interactions. The method requires minimal labor and no robotics, making it accessible for various research settings.

Keywords:
Arrayed librariesDNA–protein interactionHigh-throughputOne-hybrid systemOpen reading frameProtein–protein interactionTranscription factorsTwo-hybrid systemYeast

More Related Videos

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
07:56

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Published on: January 17, 2012

29.3K
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
14:23

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

14.2K

Related Experiment Videos

Last Updated: Feb 9, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

18.6K
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
07:56

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Published on: January 17, 2012

29.3K
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
14:23

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

14.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Molecular interactions regulate gene expression.
  • Yeast one-hybrid (Y1H) and two-hybrid (Y2H) systems detect DNA-protein and protein-protein interactions, respectively.
  • Large collections of open reading frame (ORF) clones necessitate efficient analysis protocols.

Purpose of the Study:

  • To describe a straightforward, high-throughput screening procedure for arrayed libraries using DNA or protein baits.
  • To provide a protocol adaptable for various library formats and scalable for different research needs.
  • To offer methods for preparing yeast stocks for long-term storage in tube and microplate formats.

Main Methods:

  • Development of a simple, labor-efficient protocol for high-throughput yeast hybrid screening.
  • Adaptation of the protocol for arrayed libraries and compatibility with multiple library formats.
  • Establishment of procedures for long-term yeast stock preservation.

Main Results:

  • A straightforward, high-throughput screening protocol for yeast one- and two-hybrid systems was established.
  • The protocol is designed for minimal labor, does not require robotics, and is scalable.
  • Methods for yeast stock preparation in tube and microplate formats are provided.

Conclusions:

  • The described protocol enables efficient high-throughput screening of molecular interactions using yeast hybrid systems.
  • This method is accessible to individual researchers and adaptable to various experimental scales and formats.
  • The protocol facilitates the functional analysis of large clone collections and supports long-term research endeavors.