Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.1K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.1K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.6K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.6K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.6K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.6K
Embryonic Stem Cells00:57

Embryonic Stem Cells

5.1K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
5.1K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Distinctive Features of Adult Stem Cells vs Cancer Stem Cells01:18

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

4.5K
A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Portable hepatitis C virus RNA extraction and stabilization using low-cost lab-on-a-chip style components with shelf stable reagents.

Sensors and actuators. B, Chemical·2026
Same author

United States stakeholder insights on genetic testing for equine health and breeding.

Journal of equine veterinary science·2026
Same author

Nipah virus molecular detection from whole blood and respiratory swabs in a rapid field-ready protocol.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology·2026
Same author

Charge transfer in fcc plutonium-gallium alloys.

Journal of physics. Condensed matter : an Institute of Physics journal·2025
Same author

Single-nucleus multi-omics identifies shared and distinct pathways in Pick's and Alzheimer's disease.

Science advances·2025
Same author

Validation of prototype virus inactivation from seven virus families of pandemic potential with a novel low-cost, field-deployable RNA extraction and storage method.

Journal of virological methods·2025
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 9, 2026

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
08:52

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

Published on: June 13, 2018

9.3K

Cellular Models: HD Patient-Derived Pluripotent Stem Cells.

Charlene Geater1,2, Sarah Hernandez2,3, Leslie Thompson1,2,3

  • 1Department of Psychiatry and Human Behavior, University of California-Irvine, Irvine, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 2, 2018
PubMed
Summary
This summary is machine-generated.

Human pluripotent stem cells (PSCs) offer a promising model for Huntington's disease (HD), enabling the study of patient-derived neurons. These models advance research into mutant Huntingtin (mHTT) effects and novel therapeutic strategies.

Keywords:
DifferentiationEmbryonic stem cell (ESC)Huntingtin (HTT)Huntington’s disease (HD)Induced pluripotent stem cell (iPSC)ModelingStriatum

More Related Videos

Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells
09:11

Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells

Published on: January 28, 2015

11.7K
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
10:33

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Published on: May 22, 2014

14.7K

Related Experiment Videos

Last Updated: Feb 9, 2026

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
08:52

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

Published on: June 13, 2018

9.3K
Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells
09:11

Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells

Published on: January 28, 2015

11.7K
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
10:33

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Published on: May 22, 2014

14.7K

Area of Science:

  • Neuroscience
  • Genetics
  • Stem Cell Biology

Background:

  • Huntington's disease (HD) is a neurodegenerative disorder caused by expanded polyglutamine repeats in the Huntingtin (HTT) gene.
  • Traditional HD models, including patient cells and rodent neurons, have limitations in disease manifestation and genetic context.
  • Human pluripotent stem cells (PSCs) are emerging as a valuable tool for modeling HD in patient-derived neurons and other neural cell types.

Purpose of the Study:

  • To review the use of embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) models for Huntington's disease.
  • To discuss differentiation methods for neurons from PSCs and their associated phenotypes.
  • To identify current challenges and future technical advancements in HD PSC modeling.

Main Methods:

  • Review of published literature on ESC and iPSC models for Huntington's disease.
  • Analysis of differentiation paradigms for generating neurons from PSCs.
  • Discussion of challenges in PSC and PSC-derived cell validation.

Main Results:

  • Human HD PSCs provide a platform for studying disease mechanisms in patient-derived neurons.
  • Various differentiation protocols yield neurons with specific phenotypes relevant to HD.
  • Key challenges include the validation of PSCs and their differentiated progeny.

Conclusions:

  • HD PSC models offer a human-specific system to investigate mutant Huntingtin (mHTT) effects in the central nervous system.
  • Future advances like transdifferentiation and in vitro multiorgan reconstruction hold potential for personalized medicine.
  • These models are crucial for understanding cell-type-specific consequences of mHTT and for testing novel therapies.