Fe3+-hydroxamate as immobilized metal affinity-adsorbent for protein chromatography
Journal of Chromatography
|March 8, 1985
Abstract:
The adsorbent glycinehydroxamate-Sepharose 6B, charged with Fe3+ under specified conditions, is reported. It was used at various pH values for chromatography of the following proteins: lysozyme, cytochrome c, avidin, bovine pancreatic RNase, myoglobin, ovalbumin and human serum albumin. The common naturally occurring amino acids were tested for their interactions with the new sorbent under neutral conditions.
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