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Updated: Jul 21, 2026

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
Published on: February 29, 2016
From gel filtration to adsorptive size exclusion
1Biochemical Separation Centre, University of Uppsala, BMC, Sweden.
Abstract:
Adsorption and size exclusion in starch and cross-linked dextran were phenomena discovered in Uppsala in the 1950s [Porath (1979), Biochem. Soc. Trans. 7, 1197; Porath (1981), Current Content 19, 21; Porath (1981), J. Chromatogr. 218, 241; Janson (1987), Chromatographia 23, 361; Laurent (1993), J. Chromatogr. 633, 1]. These discoveries were the background to the development of a variety of affinity chromatographic methods. At present attempts are being made to combine size exclusion chromatography (SEC) with adsorption into a single operation that we call adsorptive SEC (AdSEC).
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