Related Experiment Video
Updated: Jul 28, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Cyanocarbons as ligands for electron donor acceptor chromatography of human serum proteins
Abstract:
The adsorption of human serum proteins to mono-, di- and tri-cyanocarbon ligands was studied in the presence of the water-structuring salt Na2SO4. All of the ligands adsorbed proteins to varying degrees when coupled to agarose via a divinylsulfone-derived spacer arm (DVS), whereas an insignificant or very low protein adsorption capacity occurred when the ligands were coupled through the bisoxirane-derived spacer arm. Studies of the DVS-coupled cyanocarbons showed that adsorption capacity increases with the number of cyano substituents carried by the ligand. The selectivity toward human serum proteins appeared to be similar to that of other electron acceptor ligands, but different from that of hydrophobic ligands. Tricyanoaminopropene-DVS-agarose was found to be the most potent protein adsorbent.
More Related Videos
13:35A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
07:53Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
Published on: August 16, 2019
Related Concept Videos
Principles Of Column Chromatography
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Ion-Exchange Chromatography
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Affinity Chromatography
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...