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Myeloid cell transformation by ras-containing murine sarcoma viruses
Molecular and Cellular Biology
|April 1, 1985
Summary
Ras-containing retroviruses alter myeloid lineage cells, inducing malignant myelomonocytic cell lines. These cells differentiate into mature macrophages, showing the broad impact of ras genes on hematopoietic stem cell development.
Area of Science:
- Oncology
- Molecular Biology
- Hematopoiesis
Background:
- Ras transforming genes in BALB and Harvey murine sarcoma viruses affect erythroid and lymphoid cell lineages.
- Previous research established the role of ras genes in altering cell proliferation and differentiation.
Purpose of the Study:
- To investigate the hematopoietic targets of ras-containing viruses within the myeloid lineage.
- To characterize the properties of myeloid cells infected with ras-containing retroviruses.
Main Methods:
- Infection of murine bone marrow cells with BALB or Harvey murine sarcoma virus.
- Culture and characterization of induced colonies, including phenotypic analysis and in vitro/in vivo assays.
- Treatment of a specific cell line (BAMC1) with 12-O-tetradecanoylphorbol-13-acetate to assess differentiation.
Main Results:
- BALB or Harvey murine sarcoma virus infection induced diffuse colonies of mature macrophages with increased self-renewal capacity.
- A subset of infected cells formed a continuous, highly malignant myelomonocytic cell line (BAMC1).
- Treatment of BAMC1 with 12-O-tetradecanoylphorbol-13-acetate induced a mature myeloid phenotype with reduced growth potential, independent of p21 expression levels.
Conclusions:
- Ras-containing retroviruses impact cells at various differentiation stages within major hematopoietic lineages.
- These findings expand the understanding of ras gene targets in hematopoiesis.
- Ras genes play a significant role in altering myeloid cell growth and differentiation.