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Bacterial Coaggregation and Cohesion Among Isolates From Contact Lens Cases
Ananya Datta1, Fiona Stapleton1, Mark D P Willcox1
1School of Optometry and Vision Science, University of New South Wales Sydney, New South Wales, Australia.
Investigative Ophthalmology & Visual Science
|June 4, 2018
Summary
Bacterial coaggregation and cohesion promote colonization of contact lens cases. Specific bacteria like Staphylococcus epidermidis and Acinetobacter radioresistens showed significant interactions, aiding biofilm formation on contact lenses.
Area of Science:
- Microbiology
- Ophthalmology
- Biotechnology
Background:
- Contact lens wear is associated with microbial keratitis.
- Understanding bacterial interactions is crucial for preventing contact lens-related infections.
Purpose of the Study:
- To investigate cohesion, coaggregation, and coculture dynamics among bacteria commonly found in contact lens cases.
- To determine how these bacterial interactions influence microbial colonization.
Main Methods:
- Cohesion assays involved sequential incubation of bacteria in contact lens cases.
- Coculture experiments assessed bacterial growth inhibition over 24 hours.
- Coaggregation assays measured the ability of bacterial suspensions to aggregate, with inhibition tests using lactose, sucrose, or pronase.
Main Results:
- Initial adhesion of Micrococcus luteus or Acinetobacter radioresistens enhanced subsequent staphylococcal adhesion.
- Acinetobacter radioresistens significantly promoted Staphylococcus growth in liquid media.
- Staphylococcus epidermidis and Staphylococcus haemolyticus coaggregated with M. luteus and A. radioresistens, with interactions ranging from 30% to 54%.
Conclusions:
- Coaggregation, cohesion, and growth promotion mechanisms contribute to bacterial colonization of contact lens cases.
- These interactions may play a significant role in the pathogenesis of contact lens-related microbial keratitis.
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