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Updated: Feb 9, 2026

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
Published on: November 16, 2017
Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids
Ioana L Aanei1,2, Jeff E Glasgow1, Stacy L Capehart1
1Department of Chemistry, University of California, Berkeley, CA, USA.
Abstract:
Encapsulation into virus-like particles is an efficient way of loading cargo of interest for delivery applications. Here, we describe the encapsulation of proteins with tags comprising anionic amino acids or DNA and gold nanoparticles with negative surface charges inside MS2 bacteriophage capsids to obtain homogeneous nanoparticles with a diameter of 27 nm.
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