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|June 6, 2018
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Summary
This summary is machine-generated.

Tobacco mosaic virus (TMV) nanotubes significantly improved electrochemical glucose detection. This biosensor offers higher sensitivity, a wider linear range, and faster response times for glucose oxidase (GOD) enzyme immobilization.

Keywords:
Amperometric sensorCoat proteinEnzyme nanocarrierGlucose biosensorGlucose oxidasePt electrode arrayStreptavidin–biotinTobacco mosaic virus (TMV)

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Biosensor Development
  • Electrochemistry

Background:

  • Enzyme carriers are crucial for biosensor performance in diagnostic applications.
  • Tobacco mosaic virus (TMV) particles and coat protein (CP) aggregates offer unique structural properties for biomolecule immobilization.

Purpose of the Study:

  • To develop and evaluate a label-free electrochemical biosensor for enhanced glucose detection using TMV adapters.
  • To compare the performance of TMV nanotubes versus CP aggregates as enzyme carriers for glucose oxidase (GOD).

Main Methods:

  • Fabrication of amperometric sensor chips with platinum electrodes.
  • Immobilization of streptavidin-conjugated glucose oxidase ([SA]-GOD) onto biotinylated TMV nanotubes and CP aggregates via bioaffinity binding.
  • Comparison of [SA]-GOD immobilized via adsorption versus cross-linking with glutardialdehyde.

Main Results:

  • TMV adapters significantly enhanced amperometric glucose detection compared to CP aggregates.
  • TMV-based biosensors demonstrated superior sensitivity, an extended linear detection range, and faster response times.
  • Bioaffinity binding provided specific and efficient immobilization of [SA]-GOD complexes.

Conclusions:

  • Virus/biomolecule hybrids, specifically TMV nanotubes, show great potential for integration with electronic transducers in biosensorics.
  • TMV adapters represent a promising strategy for improving the performance of electrochemical biosensors for glucose monitoring.
  • The developed TMV-based biosensor platform is suitable for advanced biochip applications.