Related Experiment Video
Updated: Feb 9, 2026

Robot-Assisted Transcanal Endoscopic Ear Surgery for Congenital Cholesteatoma
Published on: December 15, 2023
[Study of long non-coding RNA HOTAIR expression in middle ear cholesteatoma]
1Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Nanchang University.
Abstract:
Objective:To discuss the different expression of some long-chain noncoding RNA between middle ear cholesteatoma epithelial tissue and normal external ear canal skin.Method:Real-time quantitative PCR was used to detect the expression of 6 kinds of lncRNA: HOTAIR, ANCR, TINCR, PRINS, BANCR, PICSAR in 25 cases of cholesteatoma epithelial tissues and 15 cases of normal external auditory canal skin tissue samples, respectively. And compared the expression level of lncRNA in patients with different degree of bone destruction.Result:Expression level of HOTAIR was significantly increased in cholesteatoma epithelial tissues compared with the normal external auditory canal skin tissues,and the difference is statistically significant (P< 0.01). While there was no statistically significant difference expression of the rest of the five kinds of lncRNA between middle ear cholesteatoma epithelial tissues and normal external ear canal skin (P> 0.05). And there was no statistically significant difference expression of HOTAIR in patients with different degree of bone destruction (P> 0.05).Conclusion:Expression level of HOTAIR was up-regulated in middle ear cholesteatoma epithelial tissue compared with the normal external auditory canal skin tissues, and the expression level of HOTAIR has no obvious correlation with degree of bone destruction in patients with cholesteatoma.
Related Concept Videos
lncRNA - Long Non-coding RNAs
lncRNA - Long Non-coding RNAs
What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
RNA Splicing
RNA Editing
Ribosomal RNA Synthesis
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

