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Related Experiment Video

Updated: Feb 9, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Paper Capillary Enables Effective Sampling for Microfluidic Paper Analytical Devices.

Jin-Wen Shangguan1, Yu Liu1, Sha Wang1

  • 1State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering , Nanjing University , Nanjing 210023 , People's Republic of China.

ACS Sensors
|June 7, 2018
PubMed
Summary

A novel paper capillary enhances microfluidic paper analytical devices (μPADs) for efficient, arrayed sampling. This innovation enables high-throughput, quantitative, and repeatable multiplex assays for ions, proteins, and microbes.

Keywords:
POCTmicrofluidicsmultiplex bioassaypaper analytical devicespaper capillarysampling

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Microfluidic paper analytical devices (μPADs) are valuable tools for point-of-care diagnostics.
  • Effective fluid sampling and transport remain critical challenges in μPAD design.
  • Existing μPADs can suffer from surface adsorption and component bias, limiting assay performance.

Purpose of the Study:

  • To introduce a paper capillary for improved fluid sampling in μPADs.
  • To develop a hybrid-fluid-mode paper capillary device for arrayed sampling.
  • To demonstrate the platform's capability for high-throughput, quantitative, and repeatable multiplex analysis.

Main Methods:

  • Coupling macroscale paper capillary forces with microscale native paper capillary forces to control fluid transport.
  • Designing a hybrid-fluid-mode paper capillary device for arrayed sample handling.
  • Utilizing the developed device for multiplex analysis of ions, proteins, and microbes.

Main Results:

  • The paper capillary enables flexible and tailored fluid transport.
  • The hybrid-fluid-mode device facilitates fast, reliable, and arrayed sampling with reduced surface adsorption and bias.
  • The platform supports high-throughput, quantitative, and repeatable manual assays.
  • Successful multiplex analysis of ions, proteins, and microbes was demonstrated.

Conclusions:

  • The paper capillary is a significant advancement for μPADs, enhancing sampling capabilities.
  • The hybrid-fluid-mode device offers a versatile platform for various analytical applications.
  • This technology paves the way for more sophisticated and higher-throughput analyses on μPADs.