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Related Concept Videos

Viscosity01:17

Viscosity

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When water is poured into a glass, it falls freely and quickly, whereas if honey or maple syrup is poured over a pancake, it flows slowly and sticks to the surface of the container. This difference in the flow of different kinds of liquids arises due to the fluid friction between the liquid layers and the liquid and the surrounding material. This property of fluids is called fluid viscosity. In this example, water has a lower viscosity than honey and maple syrup.
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Surface Tension, Capillary Action, and Viscosity02:57

Surface Tension, Capillary Action, and Viscosity

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Surface Tension
The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...
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Viscosity of Fluid01:19

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Viscosity measures the resistance a fluid offers to flow and deformation. It results from internal friction between layers of fluid moving relative to one another. Dynamic viscosity, denoted by the Greek letter mu (μ), quantifies the force needed to move one fluid layer over another. For Newtonian fluids like water and air, the relationship between the shearing stress and the rate of shearing strain is linear, meaning their viscosity remains constant regardless of the applied stress.
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Flow Cytometry01:23

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Labeling Emotion01:20

Labeling Emotion

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Emotional labeling is a cognitive process that involves identifying and naming one's emotions, such as anger, fear, happiness, or sadness. It allows individuals to recognize and express their internal emotional states, a critical aspect of emotional regulation and communication. Labeling emotions requires more than mere recognition; it also involves drawing upon memory and contextual cues to understand the current situation and apply a corresponding emotional label. For instance, feeling...
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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
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Pumpless Microflow Cytometry Enabled by Viscosity Modulation and Immunobead Labeling.

Byeongyeon Kim1, Sein Oh1, Suyeon Shin1

  • 1Department of Biomedical Engineering , Kyung Hee University , Yongin-si , Gyeonggi-do 17104 , Republic of Korea.

Analytical Chemistry
|June 7, 2018
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Summary
This summary is machine-generated.

This study introduces pumpless microflow cytometry (pFC), a portable, low-cost cell analysis method. It simplifies flow cytometry by removing pumps and lasers, enabling point-of-care diagnostics.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Medical Diagnostics

Background:

  • Miniaturizing flow cytometry faces challenges with bulky fluidic and optical systems.
  • Existing methods require expensive pumps and lasers, limiting portability and accessibility.

Purpose of the Study:

  • To develop a miniaturized, portable flow cytometry platform.
  • To overcome limitations of traditional flow cytometry for cell analysis.

Main Methods:

  • Utilized a viscous liquid additive for passive flow rate control, eliminating external pumps.
  • Employed an immunobead assay quantified by a portable, blue LED-based fluorescence cell counter.
  • Developed a disposable cell-counting chamber for simple sample introduction.

Main Results:

  • Achieved reliable cell counting over a dynamic range of 9-298 cells/μL.
  • Successfully identified cancer cells using CD326 (epithelial cell adhesion molecule) detection.
  • Demonstrated the platform's utility for general cell type analysis via immunobeads.

Conclusions:

  • Pumpless microflow cytometry (pFC) offers a simplified, portable, and cost-effective alternative to traditional flow cytometry.
  • The pFC platform is suitable for cell-based assays and point-of-care diagnostics.
  • This technology enables broader accessibility to cell analysis for various applications.