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Updated: Feb 9, 2026

Anaerobic Growth and Maintenance of Mammalian Cell Lines
Published on: July 21, 2018
Methods for Genomic Characterization and Maintenance of Anaerobic Fungi
Xuefeng Peng1, Candice L Swift1, Michael K Theodorou2
1Department of Chemical Engineering, University of California, Santa Barbara, CA, USA.
Abstract:
The rapid development of molecular biology and bioinformatics has fueled renewed interests in anaerobic fungi from the phylum Neocallimastigomycota. This chapter presents well-established methods for isolation, routine cultivation, and cryopreservation of anaerobic fungi. Moreover, detailed nucleic acid extraction protocols are provided, which should enable readers to isolate high-quality DNA and RNA from a variety of anaerobic fungal culture media for downstream applications such as next-generation sequencing.
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