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Electron microscopy after direct ultracentrifugation.

J Jansons, G B Harnett, M R Bucens

    Pathology
    |January 1, 1985
    PubMed
    Summary

    A novel ultracentrifugation method significantly enhances the detection of viruses like herpesviruses and poxviruses in skin lesions. This technique offers over 1,000-fold concentration, improving diagnostic accuracy for viral skin infections.

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    Area of Science:

    • Virology
    • Microscopy
    • Biotechnology

    Background:

    • Electron microscopy is crucial for diagnosing viral skin infections.
    • Conventional methods for preparing skin lesion specimens can be inefficient for virus detection.

    Purpose of the Study:

    • To introduce and evaluate a direct ultracentrifugation technique for preparing skin lesion specimens.
    • To assess the efficacy of this method in improving the detection rates of specific viruses.

    Main Methods:

    • Direct ultracentrifugation was employed for specimen preparation.
    • The concentration factor was compared to conventional adsorption techniques.
    • Detection rates of viruses were analyzed post-preparation.

    Main Results:

    • The ultracentrifugation technique achieved a concentration factor exceeding 1,000-fold.
    • This method led to a more than 300% increase in the detection rate of herpesviruses and poxviruses.
    • Enhanced viral particle concentration was observed in electron microscopy.

    Conclusions:

    • Direct ultracentrifugation is a highly effective method for preparing skin lesion specimens for electron microscopy.
    • This technique substantially improves the sensitivity for detecting herpesviruses and poxviruses.
    • The method offers a significant advancement in the diagnosis of viral skin diseases.

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