Related Experiment Video
Updated: Feb 9, 2026

Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels
Published on: July 24, 2019
Keratinocyte Motility Is Affected by UVA Radiation-A Comparison between Normal and Dysplastic Cells
Cristina M Niculiţe1,2, Marina T Nechifor3, Andreea O Urs4
1Victor Babeș National Institute of Pathology, 99-101, Splaiul Independentei, 050096 Bucharest, Romania. cristina_niculite@yahoo.com.
Abstract:
UVA radiation induces multiple and complex changes in the skin, affecting epidermal cell behavior. This study reports the effects of UVA exposure on normal (HaCaT) and dysplastic (DOK) keratinocytes. The adherence, spreading and proliferation were investigated by time-lapse measurement of cell layer impedance on different matrix proteins. Prior to UVA exposure, the time required for adherence and spreading did not differ significantly for HaCaT and DOK cells, while spreading areas were larger for HaCaT cells. Under UVA exposure, HaCaT and DOK cells behavior differed in terms of movement and proliferation. The cells' ability to cover the denuded surface and individual cell trajectories were recorded by time-lapse videomicroscopy, during wound healing experiments. Dysplastic keratinocytes showed more sensitivity to UVA, exhibiting transient deficiencies in directionality of movement and a delay in re-coating the denuded area. The actin cytoskeleton displayed a cortical organization immediately after irradiation, in both cell lines, similar to mock-irradiated cells. Post-irradiation, DOK cells displayed a better organization of stress fibers, persistent filopodia, and new, stronger focal contacts. In conclusion, after UVA exposure HaCaT and DOK cells showed a different behavior in terms of adherence, spreading, motility, proliferation, and actin cytoskeleton dynamics, with the dyplastic keratinocytes being more sensitive.
More Related Videos
10:36Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
Published on: March 23, 2022
10:03Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
Published on: January 30, 2021
Related Concept Videos
Biological Effects of Radiation
Cell Motility through Blebbing
Blebbing Through the Matrix
In multicellular...
Microtubules in Cell Motility
Actin Polymerization and Cell Motility
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
The Sense of Self: Reflected Self-Appraisal and Social Comparison
Radiation: Applications
The average...