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Flow Cytometry Purification of Mouse Meiotic Cells
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Flow Cytometry for the Immunotoxicologist.

Scott T Espenschied1, Robert M Tighe2, Kymberly M Gowdy3

  • 1Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 9, 2018
PubMed
Summary
This summary is machine-generated.

Flow cytometry offers a cutting-edge method to assess xenobiotic immunotoxicity by measuring changes in immune cell populations. This technique helps understand how toxicants impact immune responses in various organs and novel models.

Keywords:
Flow cytometryImmune cell phenotypingImmune systemImmunotoxicityZebrafish

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Area of Science:

  • Toxicology
  • Immunology
  • Flow Cytometry

Background:

  • Current regulatory testing identifies xenobiotics causing immune suppression or stimulation.
  • Understanding toxicant effects on the immune system is crucial for safety assessment.

Purpose of the Study:

  • To describe protocols for using flow cytometry to assess xenobiotic immunotoxicity.
  • To detail methods for measuring immune responses in rodent organs and novel toxicological models.

Main Methods:

  • Utilized flow cytometry to analyze immune cell populations.
  • Applied protocols across multiple rodent organs including blood, lymphoid, and nonlymphoid tissues.
  • Incorporated novel models relevant to toxicology research.

Main Results:

  • Established protocols for flow cytometry assessment of immunotoxicity.
  • Demonstrated applicability in various tissue types and emerging models.
  • Provided a framework for mechanistic studies of xenobiotic-induced immunotoxicity.

Conclusions:

  • Flow cytometry is a powerful tool for assessing xenobiotic immunotoxicity.
  • These protocols facilitate standardized testing and mechanistic investigations.
  • The methods support the evaluation of immune system responses to toxicant exposure.