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Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
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New polyacetylenes glycoside from Eclipta prostrate with DGAT inhibitory activity
Xiao Meng1, Ban-Ban Li1, Xin Lin1
1a College of Pharmacy , Beihua University , Jilin 132013 , China.
Journal of Asian Natural Products Research
|June 9, 2018
Summary
Eclipta prostrata yielded a new polyacetylene glycoside and seven known compounds. All eight compounds showed inhibitory activity against diacylglycerol acyltransferase 1 (DGAT1) in vitro.
Area of Science:
- Phytochemistry
- Pharmacology
- Enzyme Inhibition
Background:
- Eclipta prostrata is a medicinal plant with various bioactive compounds.
- Diacylglycerol acyltransferase 1 (DGAT1) plays a crucial role in triglyceride synthesis and is a target for metabolic diseases.
Purpose of the Study:
- To isolate and characterize compounds from Eclipta prostrata.
- To evaluate the inhibitory activity of these compounds on DGAT1.
Main Methods:
- Isolation of compounds using chromatographic techniques.
- Structure elucidation via spectroscopic and physico-chemical analyses.
- In vitro assay to determine DGAT1 inhibitory activity and IC50 values.
Main Results:
- One new polyacetylene glycoside, eprostrata I, and seven known compounds were isolated.
- All eight compounds demonstrated inhibitory activity against DGAT1.
- The IC50 values for the compounds ranged from 74.4 ± 1.3 to 101.1 ± 1.1 μM.
Conclusions:
- Eclipta prostrata contains compounds with significant DGAT1 inhibitory potential.
- These findings suggest Eclipta prostrata derivatives as potential therapeutic agents for DGAT1-related conditions.
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