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Transcriptomic analysis reveals the underlying pro-malignant functions of Gankyrin for colorectal cancer via

Baojin Xu1, Shenglong Li2, Wu Lv1

  • 1Department of General Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, China.

International Journal of Biological Macromolecules
|June 9, 2018
PubMed
Abstract

Insights

Gankyrin promotes colorectal cancer (CRC) progression, potentially by influencing the tumor necrosis factor (TNF) signaling pathway. This study identified BIRC3 and PSMB9 as key genes involved in Gankyrin

Area of Science:

  • Oncology
  • Molecular Biology
  • Bioinformatics

Background:

  • Gankyrin is increasingly implicated in colorectal cancer (CRC) development and progression.
  • The precise functional mechanisms of Gankyrin in CRC remain largely unelucidated.

Purpose of the Study:

  • To investigate the role of Gankyrin in colorectal cancer using microarray data.
  • To identify key genes and pathways associated with Gankyrin function in CRC.

Main Methods:

  • Utilized Gene Expression Omnibus (GEO) dataset GSE44029 for CRC microarray data.
  • Identified differentially expressed genes (DEGs) using the LIMMA method.
  • Constructed a protein-protein interaction (PPI) network and performed Gene Ontology (GO) and KEGG pathway enrichment analysis.

Main Results:

  • Identified 712 DEGs (15 upregulated, 697 downregulated) in CRC.
  • Gankyrin was found to be involved in the tumor necrosis factor-mediated signaling pathway.
  • BIRC3 and PSMB9 emerged as crucial hub genes within the Gankyrin-associated PPI network.

Conclusions:

  • Gankyrin's pro-malignant effects in CRC may be mediated through the TNF signaling pathway.
  • Altered expression of key genes, specifically BIRC3 and PSMB9, is implicated in Gankyrin's function.
Keywords:
ApoptosisColorectal cancerGankyrinTNF signaling pathway

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