Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Strong Acid and Base Solutions03:22

Strong Acid and Base Solutions

35.9K
A strong acid is a compound that dissociates completely in an aqueous solution and produces a concentration of hydronium ions equal to the initial concentration of acid. For example, 0.20 M hydrobromic acid will dissociate completely in water and produces 0.20 M of hydronium ions and 0.20 M of bromide ions.
35.9K
Weak Base Solutions03:21

Weak Base Solutions

25.3K
Some compounds produce hydroxide ions when dissolved by chemically reacting with water molecules. In all cases, these compounds react only partially and so are classified as weak bases. These types of compounds are also abundant in nature and important commodities in various technologies. For example, global production of the weak base ammonia is typically well over 100 metric tons annually, being widely used as an agricultural fertilizer, a raw material for chemical synthesis of other...
25.3K
Determining the pH of Salt Solutions04:08

Determining the pH of Salt Solutions

48.2K
The pH of a salt solution is determined by its component anions and cations. Salts that contain pH-neutral anions and the hydronium ion-producing cations form a solution with a pH less than 7. For example, in ammonium nitrate (NH4NO3) solution, NO3− ions do not react with water whereas NH4+ ions produce the hydronium ions resulting in the acidic solution.  In contrast, salts that contain pH-neutral cations and the hydroxide ion-producing anions form a solution with a pH greater than 7. For...
48.2K
Calculating pH Changes in a Buffer Solution02:45

Calculating pH Changes in a Buffer Solution

58.8K
A buffer can prevent a sudden drop or increase in the pH of a solution after the addition of a strong acid or base up to its buffering capacity; however, such addition of a strong acid or base does result in the slight pH change of the solution. The small pH change can be calculated by determining the resulting change in the concentration of buffer components, i.e., a weak acid and its conjugate base or vice versa. The concentrations obtained using these stoichiometric calculations can be used...
58.8K
Enthalpy of Solution02:39

Enthalpy of Solution

31.1K
There are two criteria that favor, but do not guarantee, the spontaneous formation of a solution:
31.1K
Chemical Reactions in Aqueous Solutions03:03

Chemical Reactions in Aqueous Solutions

72.6K
Chemical substances interact in many different ways. Certain chemical reactions exhibit common patterns of reactivity. Due to the vast number of chemical reactions, it becomes necessary to classify them based on the observed patterns of interaction.
72.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety and short-term outcomes of robotic surgery for colorectal cancer in the very elderly (≥ 85 Years): a multicenter propensity score-matched study.

Surgery today·2026
Same author

Association between surgical timing and postoperative outcome in patients with residual esophageal cancer after neoadjuvant chemoradiotherapy.

Journal of gastrointestinal oncology·2026
Same author

Short-term outcomes of robotic versus laparoscopic surgery for colon cancer: A multicenter propensity score-matched study.

Surgery today·2026
Same author

Impact of Operative Time on Postoperative Severe Complications in Elderly Patients With Colorectal Cancer: A Multicenter Retrospective Study.

Asian journal of endoscopic surgery·2026
Same author

Risk factors for one-year mortality and the subsequent prognosis in older patients undergoing curative surgery for colorectal cancer: a retrospective multicenter study.

Surgery today·2026
Same author

Oxygenated Perfusion Enhances Hepatocyte Function in Human iPSC-liver Tissue.

Transplantation·2026

Related Experiment Video

Updated: Feb 9, 2026

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor
16:45

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

Published on: December 15, 2013

10.3K

Sodium hydroxide based non-detergent decellularizing solution for rat lung.

Hideyori Sengyoku1, Tomoshi Tsuchiya1,2, Tomohiro Obata1,3

  • 1a Department of Surgical Oncology , Nagasaki University Graduate School of Biomedical Sciences , 1-7-1 Sakamoto, Nagasaki City , Japan.

Organogenesis
|June 12, 2018
PubMed
Summary

High alkaline sodium hydroxide (NaOH)-PBS solution effectively decellularized lung scaffolds, significantly reducing DNA while preserving extracellular matrix. This detergent-free method offers a cost-effective alternative for lung regeneration, comparable to traditional detergent solutions.

Keywords:
decellularizationdetergentextracellular matrixlung regenerationsodium hydroxide

More Related Videos

Engineered Lung Tissues Prepared from Decellularized Lung Slices
08:01

Engineered Lung Tissues Prepared from Decellularized Lung Slices

Published on: January 21, 2022

4.1K
Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells
10:13

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

794

Related Experiment Videos

Last Updated: Feb 9, 2026

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor
16:45

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

Published on: December 15, 2013

10.3K
Engineered Lung Tissues Prepared from Decellularized Lung Slices
08:01

Engineered Lung Tissues Prepared from Decellularized Lung Slices

Published on: January 21, 2022

4.1K
Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells
10:13

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

794

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science

Background:

  • Lung transplantation is limited by donor organ shortage.
  • Lung regeneration via decellularized scaffolds is a promising alternative.
  • Detergents are commonly used for decellularization, but alternatives are explored.

Purpose of the Study:

  • To evaluate a high alkaline sodium hydroxide (NaOH)-PBS solution for lung decellularization.
  • To compare its efficacy with detergent-based solutions (CHAPS, SDS) regarding DNA elimination and extracellular matrix (ECM) preservation.
  • To assess the recellularization potential and ex vivo lung function of NaOH-treated scaffolds.

Main Methods:

  • Decellularization of lung scaffolds using NaOH-PBS (pH 12) without detergents.
  • Comparison with CHAPS and SDS decellularization solutions.
  • Immunohistochemical analysis for cell component removal.
  • Collagen and glycosaminoglycan (GAG) assays for ECM preservation.
  • Human Umbilical Vein Endothelial Cell (HUVEC) recellularization.
  • Ex vivo functional analysis of oxygen ventilation.

Main Results:

  • NaOH solution effectively removed cell components, comparable to CHAPS and SDS.
  • Collagen reduction was similar across all groups.
  • DNA reduction was significantly greater with NaOH (p < 0.0001).
  • HUVEC cell attachment was not inferior to the SDS group.
  • Regenerated lungs showed functional oxygen ventilation in both SDS and NaOH groups.

Conclusions:

  • NaOH-PBS is a viable, detergent-free method for lung decellularization.
  • It offers significant DNA reduction and comparable ECM preservation.
  • This approach is cost-effective with negligible residues, presenting a potential alternative for clinical lung regeneration.