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Related Concept Videos

Coronary Circulation01:21

Coronary Circulation

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The heart, an organ critical to survival, gets nourishment not from the blood it pumps but from a separate circulation system known as coronary circulation. This is the shortest circulation in the body and is responsible for supplying the heart with the nutrients it needs to function effectively.
Coronary circulation begins at the base of the aorta, where two main arteries arise—the left and right coronary arteries. These arteries encircle the heart in the coronary sulcus and supply the...
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Fetal Circulation01:14

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Fetal circulation is a unique system that facilitates the exchange of gases, nutrients, and waste products between the developing fetus and the mother. This intricate process takes place through a special organ called the placenta.
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Overview of Pulmonary Circulation01:19

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The pulmonary circulation is a vital system in our body that acts as a bridge between the respiratory and cardiovascular systems. It serves as a transport network for deoxygenated blood from the heart to the lungs and then returns oxygen-rich blood back to the heart.
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Overview of Systemic and Pulmonary Circulation01:15

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The systemic and pulmonary circuits are crucial components of the circulatory system, working together to transport blood between the heart, lungs, and the rest of the body. The process begins with pulmonary circulation, where deoxygenated blood is pumped from the right ventricle to the lungs via the pulmonary trunk and arteries. Upon reaching the lungs, the blood becomes oxygenated and returns to the heart, specifically to the left atrium, via the pulmonary veins.
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Cancer Stem Cells and Tumor Maintenance02:40

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Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
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Mass Analyzers: Overview01:13

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The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
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Capture and Release of Viable Circulating Tumor Cells from Blood
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Analyzing Circulating Tumor Cells One at a Time.

Veronica Ortiz1, Min Yu1

  • 1Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.

Trends in Cell Biology
|June 13, 2018
PubMed
Summary
This summary is machine-generated.

Single-cell sequencing reveals rare cancer cell details missed by bulk methods. This review explores techniques for analyzing circulating tumor cells (CTCs) and their limitations.

Keywords:
Circulating tumor cellssingle-cell sequencingtumor heterogeneity

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Area of Science:

  • Genomics
  • Cancer Biology
  • Molecular Oncology

Background:

  • Whole-genome sequencing revolutionized cancer research but struggles with rare cell detection.
  • Bulk sequencing methods provide limited insights into cancer cell heterogeneity.
  • Single-cell sequencing (SCS) offers unprecedented resolution for studying cancer progression and diversity.

Purpose of the Study:

  • To review various single-cell sequencing techniques applicable to circulating tumor cells (CTCs).
  • To elucidate the molecular properties of CTCs using advanced sequencing methodologies.
  • To identify and discuss the limitations of current SCS methods for CTC analysis.

Main Methods:

  • Review of existing literature on single-cell sequencing technologies.
  • Analysis of methods for isolating and analyzing circulating tumor cells (CTCs).
  • Comparative assessment of different SCS techniques for their utility in cancer research.

Main Results:

  • SCS methods provide detailed molecular profiles of individual cancer cells, including CTCs.
  • Various SCS techniques offer distinct advantages for characterizing CTCs.
  • Current SCS methods face challenges such as technical limitations and cost.

Conclusions:

  • Single-cell sequencing is crucial for understanding rare cell populations like CTCs.
  • Further development is needed to overcome existing drawbacks in SCS for comprehensive CTC analysis.
  • Advancements in SCS will enhance our understanding of cancer metastasis and inform therapeutic strategies.