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Adenosine receptors on human inflammatory cells.

G Marone, R Petracca, S Vigorita

    International Archives of Allergy and Applied Immunology
    |January 1, 1985
    PubMed
    Summary

    This study explores how adenosine, a natural nucleoside, affects immune cells. It identifies specific receptors on B and T lymphocytes, neutrophils, and other inflammatory cells. The researchers found that activating A2/Ra receptors increases cyclic AMP levels, while A1/Ri receptors and P-sites inhibit responses to various agonists like isoproterenol and histamine. These findings suggest adenosine plays a role in modulating immune cell activity through receptor interactions. The study contributes to understanding how adenosine influences immune signaling pathways.

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    Area of Science:

    • Immunology
    • Pharmacology
    • Cell signaling

    Background:

    Adenosine is known to influence immune cell activity, but specific roles of its receptors remain unclear. Prior research has shown adenosine modulates cyclic AMP levels in various cells. However, the exact distribution of adenosine receptors on inflammatory cells is not fully mapped. This gap motivated investigation into receptor types and their functional roles. No prior work had resolved the presence of A1/Ri and P-sites on multiple cell types. The literature suggests adenosine receptors affect immune responses. That uncertainty drove the need for a detailed receptor analysis. This study aimed to clarify receptor distribution and function on human inflammatory cells.

    Purpose Of The Study:

    The study aimed to identify adenosine receptor subtypes on human inflammatory cells. It focused on B and T lymphocytes, neutrophils, monocytes, and others. The researchers wanted to determine if these cells express A2/Ra receptors. They also sought evidence of A1/Ri receptors and P-sites. The goal was to assess how receptor activation affects cyclic AMP levels. This work sought to clarify the physiological role of adenosine in immune cells. The motivation was to better understand immune modulation mechanisms. The study aimed to provide a detailed receptor profile for these cell types.

    Keywords:
    Adenosine receptorsImmune cell signalingHuman lymphocyte receptorsCyclic AMP modulation

    Frequently Asked Questions

    Activation of A2/Ra receptors increases intracellular cyclic AMP levels in these cells.

    P-site activation inhibits responses to isoproterenol, PGE1, histamine, and adenosine.

    A1/Ri receptors mediate inhibitory effects on cyclic AMP levels in these cells.

    B and T lymphocytes, neutrophils, monocytes, basophils, and platelets were tested.

    Cyclic AMP levels were measured after receptor activation using biochemical assays.

    Related Experiment Videos

    Main Methods:

    The researchers used membrane preparations from human inflammatory cells. They tested for the presence of adenosine A2/Ra receptors using binding assays. Cyclic AMP levels were measured after receptor activation. The study also examined inhibitory A1/Ri receptors and P-sites. Functional assays assessed receptor responses to agonists like isoproterenol. Cell types included B and T lymphocytes, neutrophils, monocytes, and platelets. The methods involved biochemical analysis and receptor stimulation. The approach combined receptor identification with functional evaluation.

    Main Results:

    Human B and T lymphocytes, neutrophils, and others express A2/Ra receptors. Activation of these receptors increased intracellular cyclic AMP levels. The study found A1/Ri receptors and P-sites on lymphocytes and neutrophils. P-site activation inhibited responses to isoproterenol and PGE1. The inhibitory effect was also observed with histamine and adenosine. Cyclic AMP levels dropped after P-site stimulation. The results suggest adenosine modulates immune cell signaling. The findings confirm receptor presence and functional roles in these cells.

    Conclusions:

    The authors propose that adenosine receptors modulate immune cell activity. They suggest A2/Ra receptors increase cyclic AMP in inflammatory cells. The presence of A1/Ri receptors and P-sites was confirmed in lymphocytes. These receptors inhibit responses to various agonists, including isoproterenol. The findings support a role for adenosine in immune regulation. The study suggests P-sites contribute to cyclic AMP inhibition. The authors state these receptors are functionally active in immune cells. The results align with prior evidence of adenosine’s physiological role.

    P-sites suggest adenosine can modulate multiple signaling pathways in immune cells.